Adenosine Diphosphate as the Primary Phosphoryl Acceptor in Oxidative Phosphorylation
نویسندگان
چکیده
منابع مشابه
Inorganic orthophosphate activation and adenosine diphosphate as the primary phosphoryl acceptor in oxidative phosphorylation.
Experiments are reported showing that in net oxidative phosphorylation by submitochondrial particles ADP furnishes the terminal bridge oxygen in the ATP formed. In contrast to ADP, AMP does not suffice to activate a inorganic orthophosphate -F? HOH exchange by submitochondrial particles. In sensitive initial labeling experiments during net oxidative phosphorylation, “Pi appears initially in ATP...
متن کاملThe adenosine triphosphate-adenosine diphosphate exchange reaction of oxidative phosphorylation.
Previous reports from this laboratory have demonstrated that the ATP-Pi32 1 exchange reaction (1) and the dinitrophenolstimulated ATPase activity (2) of mitochondrial subfragments (3-9) are reflections of intermediate enzymatic steps in the complex sequence of reactions (1, 3) in which phosphorylation of ADP is coupled to electron transport in the respiratory chain. This communication concerns ...
متن کاملFree adenosine diphosphate as an intermediary in the phosphorylation by creatine phosphate of adenosine diphosphate bound to actin.
Pretreatment of adenosine diphosphate-G-actin solutions with apyrase results in a marked decrease in their rate of polymerization at 0” induced by KCl, creatine phosphate, and creatine kinase. In contrast, apyrase has no effect on the rate of polymerization of ATP-G-actin. The retardation can be reversed by the addition of a low concentration of ADP or of ATP. By comparing the rate of polymeriz...
متن کاملThe apparent absolute requirement of adenosine diphosphate for the inorganic phosphate--water exchange of oxidative phosphorylation.
With suitable submitochondrial particles from the heart, the rate of the inorganic phosphate e HOH exchange during substrate oxidation in the absence of ADP is less than l/1000 of the rate in the presence of ADP. The concentration dependence of the exchange on ADP shows simple saturation behavior with an apparent K, of 0.32 mM. For net oxidative phosphorylation under similar conditions, but wit...
متن کاملThe affinity of mitochondrial oxidative phosphorylation mechanisms for phosphate and adenosine diphosphate.
There is now increasing evidence for the occurrence of specific carrier systems in the mitochondrial membrane that permit certain metabolites to cross it freely, possibly by exchange-diffusion mechanisms.1-5 One of these systems is specific for adenosine diphosphate (ADP) and adenosine triphosphate (ATP) and it appears to be the site at which atractyloside inhibits a number of mitochondrial rea...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1969
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)78202-7